Costimulatory molecules and adhesion molecules in experimental colitis of rats / 中国免疫学杂志

ABSTRACT

Objective:To investigate costimulatory molecules and adhension molecules in the pathogenesis of experimental colitis in rats.Methods:Models of experimental colitis were established by enema with trinitrobenzenesulphonic acid. Prednisolone or salicylazosulfapyridine was given as treatment.Nomal rat and untreated colitis rat groups were set up as controls.Histological scoring was performed.Expression of CD11b,CD28 and CTLA-4 were detected by flow cytometry in peripheral blood,spleen and intraepithelial mononuclear cells of colon.Results:Expressions of CD11b,CD28 and CTLA-4 were high in experimental colitis. CD11b and CD28 decreseased after treatment of prednisolone or salicylazosulfapyridine,while CTLA-4 increased further,which were significant statistically.Conclusion:Adhesion molecules may have a role in the pathogenesis of experimental colitis.The dysregulation of CD28 and CTLA-4 may be one of the mechanisms in IBD.The mechanisms of prednisolone and salicylazosulfapyridine may be associated with costimulatory molecules and adhension molecules.