Determination of icariin in Yinyangsu capsules by HPLC / 西安交通大学学报(医学版)

ABSTRACT

Objective To establish an HPLC method for testing the content of icariin in Yinyangsu capsules.Methods An Lichrospher C18(4.6mm?250mm,5?m) was used as the column,and column C18 as the protecting column.The mobile phase was a mixture of acetonitrile-20g/L acetum(30∶70) in a flow rate of 1.0mL/min.The detected wavelength was 270nm.Column temperature was room temperature and the injection quantity was 10?L.The sample was prepared when we added 50%(V/V) alcohol and then made ultrasonic extraction.Measurement was made after the prepared sample was filtrated on 0.45?m filter paper.Results Icariin in the sample was well separated.The linear range was 10.0-60.0mg/L.The linear equation was C=2.042?10-5A+0.554,while the correlation coefficient was 0.9993;the average recovery was 99.8% and RSD was 0.65%(n=6).Conclusion This method can be used in content measurement of Yinyangsu capsules.