Experiment study of the role of allogeneic serum in the culture of human bone marrow mesenchymal stem cells in vitro / 中国矫形外科杂志

ABSTRACT

[Objective]To investigate if allogeneic human serum(alloHS)could replace fetal bovine serum(FBS)for the expansion of human bone marrow mesenchymal stem cells(hBMSCs)in vitro.[Method]Human BM-MSCs were isolated either from spongy bone residues obtained during hip surgery or from washed filters used during bone marrow graft processing for allogeneic bone marrow transplantation.We culture human bone marrow mesenchymal stem cells were cultivated using alloHS and FBS,then observe its growth character,antigen presentation and differentiation potency through condition medium directional induction were observed.[Result]Isolated MSCs were positive for the markers CD105,CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34,CD45,CD106 and HLA-DR.The choice of serum affected hMSCs at several different levels.Firstly,hMSCs in alloHS proliferated markedly faster than hMSCs in FBS.Secondly,hMSCs in FBS differentiated more rapidly toward mesenchymal lineages compared with hMSCs in alloHS.[Conclusion]hMSCs may be expanded rapidly in alloHS in the absence of growth factors,this may be a safely culture method suitable for clinical demand.