Effects of Tripterygium Wlilfordii on renal interstitial fibrosis and inflamed lesion in UUO mice kidney / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effects of Tripterygium Wlilfordii on renal interstitial fibrosis and the expression of inflammatory factor in mice kidney with the Unilateral Ureteral Obstruction(UUO).Methods:Mice were randomly assigned to shame operation group(shame proup), UUO group, Tripterygium Wlilfordii and ACEI treatment group after UUO. The protein expression of ?-SMA,ICAM-1 were assessed by immunohistochemistry or Western blot; MCP-1 mRNA were detected by reverse transcription-polymerase chain reaction analyses(RT-PCR). And the change of renal pathology was observed at the same time.Results:In comparison with the shame group, the protein levels of ?-SMA, ICAM-1 and the mRNA expression of MCP-1 in UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression of ?-SMA, ICAM-and MCP-1 on levels of protein and gene. The effects of Tripterygium Wlilfordii on ICAM-1 and MCP-1 were more significant than ACEI.Conclusion:Tripterygium Wlilfordii could reduce the transdifferentiation of renal interstitial cells and prevent renal interstitial inflammatory injury.