Radiation Effect on NO, NOS and TGF-beta Expressions In Rat Lung / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
;
: 321-328, 2000.
Article
in Korean
| WPRIM
| ID: wpr-54492
ABSTRACT
PURPOSE:
NOS2 induce NO production and NO activate TGF-beta. The TGF-beta is a inhibitor of NOS2. If this negative feedback mechanism operating in radiation pneumonitis model, NOS2 inhibitor may play a role in TGF-beta suppression. We planned this study to evaluate the expression patterns of NO, NOS2 and TGF-beta in vivo radiation pneumonitis model. MATERIALS ANDMETHODS:
Sixty sprague-Dawley rat were irradiated 5 Gy or 20 Gy. They were sacrificed 3, 7, 14, 28 and 56 days after irradiation. During sacrifice, we performed broncho-alveolar lavage (BAL). The BAL fluids were centrifuged and supernatents were used for measure NO and TGF-beta, and the cells were used for RT-PCR.RESULTS:
After 5 Gy of radiation, NO in BAL fluid increased at 28 days in both lung and TGF-beta in left lung at 56 days. NO increased in BAL fluid at 28 days in both lung after irradiation and TGF-beta in right lung at 28-56 days after 20 Gy of radiation. After 5 Gy of radiation, NOS2 expression was increased in right lung at 14 days, in both lung at 28 days and in left lung at 56 days. TGF-beta expression was reduced in both lung at 28 days and increased in left lung at 56 days.CONCLUSIONS:
The proposed feedback mechanism of NO, NOS2 and TGF-beta was operated in vivo radiation pneumonitis model. At 56 days, however, NOS2 and TGF-beta expressed concurrently in left lung after 5 Gy and in both lung after 20 Gy of radiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Transforming Growth Factor beta
/
Rats, Sprague-Dawley
/
Radiation Pneumonitis
/
Therapeutic Irrigation
/
Lung
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Year:
2000
Type:
Article
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