Effects of arecoline on the expression of human telomerase reverse transcriptase in oral mucosa keratinocytes / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the effects of arecoline on the expression of human telomerase reverse transcriptase(hTERT)mRNA and protein in cultured normal human oral mucosa keratinocytes(KCs),and then to investigate the role of hTERT in carcinogenesis of oral submucous fibrosis(OSF).Methods:Normal human oral mucosa KCs were cultured in vitro,and in experiment group,KCs was divided into 0.03,0.06,0.09 g/L arecoline group,and 0.0 g/L arecoline group served as the control.The hTERT mRNA and protein expression of KC was examined by reverse transcription polymerase chain reaction(RT-PCR)and Western blotting.Results:Arecoline induced the hTERT mRNA and protein expression of KC in a dose dependent manner.hTERT mRNA and protein expression of KC were increased by 0.03,0.06 and 0.09 g/L arecoline,when compared with the control group(P