Observation of Nam Dinh virus multiplication on Aedes albopictus cell line clone C6/36 by electron microscopy

ABSTRACT

Background: Virus is one of main causes of children acute encephalitis syndrome in countries of Asia south-east. In Vietnam, apart from Japanese Encephalitis Virus which is considered as main cause of children acute encephalitis syndrome, there are other viral pathologies, of which is Nam Dinh virus. Nam Dinh virus \ufffd?a novel Arbovirus was isolated from acute encephalitis syndrome patient in northern Vietnam, 2002. The circulation of this virus has been recognized in the north, central, and highland regions. Objective: To observe the multiplication of Nam Dinh virus on the Aedes albopictus cell line clone C6/36. Materials and method: In this study the ultra-thin section method was used to observe the multiplication of the Nam Dinh virus on the Aedes albopictus cell line clone C6/36, 48 hours post-infection. Results and Conclusion: Nam Dinh encephalitis virus got used to Aedes albopictus cell line clone C6/36 and damaged cells, 24-48 hours post-infection. Its multiplication is taking place in the cytoplasm, a typical characteristic of RNA virus. Nucleocapsids of the virus were found in vacuoles of the cell. Proteincapsid of the virus was synthesized in a rough endoplasmic reticulum (rER). After assembling in the cytoplasm, the virus is released from the cell by budding and used the cell membrane as its envelope.