Effect of acitretin in inducing the expression of heparin-binding epidermal-growth-factor-like growth factor mRNA in normal human keratinocytes / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the alteration of cell proliferation and heparin-binding epidermal-growth-factor-like growth factor(HB-EGF)mRNA expression in cultured normal human keratinocytes after acitretin treatment.Methods After a 12-hour incubation with 0.1 and 1 ?mol/L acitretin in normal human keratinocytes,the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HB-EGF mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).Results Both keratinocytes growth inhibition and HB-EGF mRNA expression was dose-dependently induced by acitretin.Treatment with 0.1 and 1 ?mol/L acitretin inhibited keratinocyte proliferation by 10.2% and 14.4%,and elevated HB-EGF mRNA by 3.2-and 7.1-fold,respectively.Conclusion Upregulated HB-EGF mRNA expression induced by acitretin in normal human keratinocytes may be involved in regulating keratinocyte growth inhibition after acitretin treatment.