Apoptosis of nasopharyngeal cancer cells line CNE2 induced by diallyl disulfide and its possible molecular mechanism / 中国癌症杂志

ABSTRACT

Background and purpose:Diallyl disulfide (DADS) is an oil-soluble organosulfur compound found in garlic. It can induce apoptosis in various cancer cell lines in vitro. This study investigated the effect of apoptosis of nasopharyngeal cancer cells line CNE2 induced by diallyl disulfide (DADS) and its molecular mechanisms. Methods:The growth inhibition and apoptosis of CNE2 cells and the protein levels of Bcl-2, Bax and Caspase 3 were examined by MTT assay, light microscopy, flow cytometry, DNA agarose gel electrophoresis and Western blot, respectively.Results:MTT assay showed that 50,100,200,400 ?mol/L DADS significantly inhibited proliferation of CNE2 cells at 48 hr, its inhibition ratio were 3.66%, 14.28%, 29.66%, 61.28%, respectively, in dose-dependent manner (P