Effect of Tie-2-siRNA Expression Vector on Human Hepatoma Transplanted Subcutaneously in Nude Mice / 中国普外基础与临床杂志

ABSTRACT

Objective To explore the effect of Tie-2 small interference RNA(siRNA)treatment in human hepatoma transplanted subcutaneously in nude mice.Methods Tumor cells were implanted in the hind flank of male nude mice of 6 weeks.Tumor-bearing mice were divided into two groups(gene therapy group and control group)and injected intra-tumorally with Tie-2-siRNA/Lipofectamine and saline/Lipofectamine respectively.The tumor volume and weight,serum AFP and microvessel density(MVD)and the histological change of the tumor were tested after gene therapy.Results The growth inhibitory rates in gene therapy group were 26.94%,53.01% and 68.91% on day 4,7 and 10 after gene therapy respectively.The tumor volumes of gene therapy group(118.47,111.57 and 104.59 mm3)were smaller than those of the control group(162.17,237.46 and 336.41 mm3)respectively(P