Construction of eukaryotic expression carrier of recombinant pcDNA3.1-hBMP-7 and transfection into bone marrow stromal cells of rabbit / 中国矫形外科杂志
Orthopedic Journal of China
;
(24)2006.
Article
in Chinese
| WPRIM
| ID: wpr-545546
ABSTRACT
[Objective]To construct arecombinant eukaryotic expression plasmid carrying human BMP-7 gene which was transfected into bone marrow stromal cells(BMSCs) in vitro and expressed.[Method]Clone human BMP-7 cDNA from a Chinese woman placenta was recombinanted with plasmid pcDNA3.1,to construct eukaryotic expression carrier of recombinaut pcDNA3.1-hBMP-7.The BMSCs were isolated from rabbit bone marrow and cultured in vitro.They were divided into three groups pcDNA3.1-hBMP-7 transduced group;pcDNA3.1 transduced group;untransduced group.The expression of hBMP-7 was detected by RT-PCR,Immuneohistochemistry.ALP,Collogen,Osteocalcin production were detected.[Result]The pcDNA3.1-hBMP-7 transduced BMSCs expressed hBMP-7 at both mRNA and protein levels.ALP activity was detected in pcDNA3.1-hBMP-7 transduced cells from day 2 to day 10,peaking on day 8.Collogen,osteocalcin production were elevated significantly too(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Orthopedic Journal of China
Year:
2006
Type:
Article
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