The purification of anti-D antibody from IgG contained anti-D by the RhD positive red blood cell / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-545674
ABSTRACT
Objective:
To investigate the purified methods of human anti-D antibody from IgG contained anti-D.Methods:
The IgG was separated by the column ion-exchange chromatography(CIEC) from the plasma in which the content of anti-D was 0.814 ?g/ml. Then the IgG preparation contained anti-D was purified by the affinity chromatography(AC) with the O group, RhD positive red blood cell (genotype CCDee).Results:
The content of non anti-D IgG were reduced about 90% by the method of AC and the proportion of anti-D could be significantly increased in the final preperation. The quality of final preparation attained reqirements of national standard of biologics.Conclusion:
This method is able to purify anti-D from IgG contained anti-D and offer a reference for plasma products.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS