Effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line and MEK/ERK, NF-?B signaling pathway / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-545698
ABSTRACT
Objective:
To investigate the effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma cell ACC-2 in vitro.Methods:
The effect of bFGF on proliferation of ACC-2 cell line was observed by MTT assay; ERK activity was measured by immuno-precipitation; and ERK and I-?B? expressions were assessed by Western blot.Results:
bFGF can enhance the proliferation of ACC-2 cell line. Stimulated by bFGF, the proliferation ratio increased significantly. The intracellular ERK activity and p-ERK expression were increased and I-?B? expression was inhibited by different concentrations of bFGF. The above effects of bFGF can be attenuated by MEK inhibitor U0126.Conclusion:
bFGF stimulates the proliferation of ACC-2 in the dose dependent manner, which may be due to up-regulating ERK, NF-?B signaling pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2000
Type:
Article
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