Determination of Nicotine in Tobacco by High Performance Liquid Chromatography / 环境与健康杂志
Journal of Environment and Health
;
(12)1993.
Article
in Chinese
| WPRIM
| ID: wpr-545897
ABSTRACT
Objective To develop a high performance liquid chromatography (HPLC) method for the determination of nicotine in tobacco. Methods The nicotine was separated completely by C18 column (column temperature∶35℃ ), and was determined by PDA detector at wave length of 260 nm. Mobile phase was acetonitrile 0.08 mmol/L KH2PO4 (V∶V= 1∶9) at the flow rate of 1.0 ml/min. Results The detection limits is 0.40 ?g/ml. The linear range was 0.00-60.0 ?g/ml. The recovery rates were 97.8%-103% and the relative standard deviations was 2.29% (n=5). Conclusion The method was simple, rapid, sensitive and accurate for the determination of nicotine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Environment and Health
Year:
1993
Type:
Article
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