Plasticity of 5-HT receptors in the IBS subgroups of experimental rats / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the effect of 5-hydroxytryptamine(5-HT) and the plasticity of 5-HT3 and 5-HT4 receptors in the myenteric plexus on the pathogenesis of irritable bowel syndrome(IBS) subgroups.Methods Twenty-seven male rats were randomly divided into three groups IBS with diarrhea(IBS-D) group,IBS with constipation(IBS-C) group and blank control group.IBS-D model was made in rats by intracolonic instillation with acetic acid.IBS-C model was made in rats by gastric instillation with cool water.After rats in each group were killed,a colon segment was removed for detecting enteric neuron and excitatory neurotransmitter in the myenteric plexus using 5-HT and 5-HT3,5-HT4 receptors immunohistochemistry method.Results ① The number of 5-HT positive neurons in the myenteric plexus of IBS-D group(4.37?0.88) group was significantly higher than that in control group(2.99?0.41,P0.05).② The results of the experiment showed that there was no difference in the number of 5-HT3 receptor positive neurons in the myenteric plexus among each group(F=0.082,P=0.922).③ The positive value of 5-HT4 receptor immunoreactive neurons in the myenteric plexus of IBS-D group(141.98?6.96) group was significantly higher than that in control group(159.90?2.55)(P0.05).Conclusion Increased number of 5-HT and 5-HT4 receptor positive neurons in the myenteric plexus may be related to the pathogenesis of IBS-D rat model.