Effect of Cold Stress on Secretory Function of Luteal and Granulose Cells of Rat in vitro / 环境与健康杂志

ABSTRACT

Objective In order to study the effect of cold stress on the secretory function of rat ovary, the changes of hCG-induced progesterone and cAMP were observed and the livability of the oocytes was determined. Methods The rat luteal and granulose cells and the oocytes were cultured, progesterone and cAMP content was determined by radioimmunoassay kit (RIA kit) under 37 ℃, 0 ℃, -5 ℃, -10 ℃,-15 ℃, -20 ℃ and -25 ℃ respectively. The livability of the oocytes was determined by MTT assay. Results At -5 ℃ to -25 ℃, the content of progesterone in the luteal cells and granulose cells medium decreased compared with the control group. At 0 ℃～-25 ℃, the content of cAMP in the luteal cells and granulose cells medium was higher compared with the control. The livability of the oocytes was decreasing from -15 ℃. Conclusion Cold stress can inhibit hCG-induced progesterone secretion in the luteal and granulose cells of rats in the one hand, and can increase cAMP, decrease the livability of the oocytes in the other hand.