Effects of Long-term Consumption of Purified Water on Lead Accumulation in Organs of Rats / 环境与健康杂志

ABSTRACT

Objective To understand whether long term consumption of purified water can cause lead accumulation and enhance lead toxicity in the rats with chronic lead exposure. Methods 104 male SD weaned rats were randomly divided into eight groups,tap water,purified water,tap water plus lead (lead acetate,Pb2+ 50 mg/L ),purified water plus lead (Pb2+ 50 mg /L),tap water plus lead (Pb2+ 200 mg/L ),purified water plus lead (Pb2+ 200 mg/L),tap water plus lead (Pb2+ 800 mg/L),purified water plus lead (Pb2+ 800 mg/L). All were fed with normal food and kept in the same environmental conditions. The blood samples were collected after 4,6,8,10,24 and 28 weeks of lead exposure. The brain,heart,liver,kidney,bone were sampled at the experimental endpoint and the lead concentration was determined with graphite furnace atomic absorption spectrometric method,zinc protoporphyrin (ZPP) level was measured by using surface fluorescence method. Results At the same lead exposure level,no difference of blood lead level was observed between the groups of drinking purified water and tap water,however,the lead level in the organs tissue,including brain,heart,liver,kidney,bone,was significantly higher in the group drinking purified water compared with drinking tap water. The blood ZPP level in rats drinking purified water was also higher than the rats drinking tap water,the significant difference were occurred at low lead level exposure (P