In vitro evaluation of Ti-24Nb-4Zr-7.9Sn coated with microarc-oxidation film in cultured osteoblast cells / 实用口腔医学杂志

ABSTRACT

Objective: To study the attachment, proliferation and ALP activity of osteoblasts on Ti-24Nb-4Zr-7.9Sn(TNZS) alloy surface treated by microarc oxidation (MAO). Methods: The surface roughness and energy of TNZS before and after MAO treated was examined by physicochemical methods, and pure titanium used as control. The primary cultured osteoblasts separated from calvarium of fetal rats were cultured and the third passage osteoblasts were seeded on 3 different surfaces of Ti, TNZS, MAO-TNZS discs. Biological assays were performed by MTT method. All data were statistically analyzed. Results: The surface energy and roughness of MAO-TNZS was higher than that of other groups; during the initial period of the cell adhesion on the materials, there were no differences among the three teams. But after 2 h, the cells adhesions on the surface of Mao-TNZS were higher than that on smooth surface and Ti (P