Roles of NF-?B in expression of hBD-2 mRNA in human primary epithelial cells induced by TNF-? / 第三军医大学学报

ABSTRACT

Objective To explore the roles of NF-?B in expression of human ?-defensin-2(hBD-2) mRNA induced by TNF-? in the human airway primary epithelial cells.Methods After the human bronchial primary epithelial cells were stimulated with TNF? or first with NF-?B inhibitor PDTC,then TNF-?,the expression of hBD-2 mRNA was detected by RT-PCR.The I?B-? protein level in the cytoplasm was detected by Western blotting and the nuclear factor-kappa B(NF-?B) binding activity was analyzed by electrophoretic mobility shift assays.Results The hBD-2 mRNA could be detected after 2.5 h of TNF-? stimulation and expressed in a dose-dependent manner.The NF-?B could be activated after 0.5 h of TNF-? stimulation.The supershifts assays indicated that the p65-p50 heterodimer formed complexes of NF-?B were involved in the activated of NF-?B.Conclusion TNF-? can induce the expression of hBD-2 mRNA in a dose-dependent manner.The p65-p50 heterodimer formed complexes of NF-?B play an important role in the regulation of hBD-2 gene expression in response to TNF-?.