Effect of Vitamin E on advanced glycosylation end-products and their receptors in renal tissue of diabetic rats / 西安交通大学学报(医学版)

ABSTRACT

Objective To observe the effect of Vitamin E on advanced glycosylation end-products(AGEs) and their receptors in renal tissue of diabetic rats.Methods Rat diabetic nephropathy was induced by intraperitoneal injection of STZ.Rats were allocated to normal control group(NC group),diabetes mellitus group(DM group),and Vitamin E group(VE group).All rats were treated with corresponding drugs for 8 weeks.During and after the treatment,the general state,blood glucose level(BGL),blood urea nitrogen(BUN),serum creatinine(Scr),urinary albumin excretion rate(UAER),glycosylated hemoglobin(HbA1C),clearance rate of creatinine(Ccr) and kidney weight/body weight ratio were determined in different groups.The fluorescence microscope was used to observe advanced glycosylation end-products fluorescence intensity in iced slices.The expression of RAGE in rats' renal tissue slices was measured by immunohistochemistry.Results ① Compared with those in NC group,GLU,HbA1C,BUN,UAER,kidney weight/body weight ratio,contents of AGEs,and RAGE increased significantly(P