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In vitro co-culcure of mouse osteoblasts combined with magnesium alloys / 中国矫形外科杂志
Orthopedic Journal of China ; (24)2006.
Article in Chinese | WPRIM | ID: wpr-546516
ABSTRACT
[Objective]To observe mouse osteoblasts vitality,histomorphology and growing development in vitro co-culture combined with magnesium alloys,confirm the biocompatibility of magnesium alloys and osteoblasts,and try to find out the possibility of magnesium alloys to be a new type of bone surgery implant material.[Method]Mouse osteoblasts were checked by Gomori and Von kossa stained,cultured and developed in vitro.Then the osteoblasts were mixed with magnesium alloys in a cell density of 3?104/ml.After 24、48、72 hours co-culture,the surface of magnesium alloys were observed by scanning electron microscopy(SEM) and confocal microscopy to find the change of osteoblasts.[Result]After culture in vitro,the osteoblasts well developed,and expressed stable character.After co-culture with magnesium alloys,the cells adhered and proliferated on the surface of alloys very well from SEM and confocal microscopy observation,which showed the osteoblasts had great activity and reproduce capability.[Conclusion]Magnesium alloys showed good biocompatibility and bone conduction capability with mouse osteoblasts,so the magnesium alloy is very possible to be a new type of bone surgery implant material.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Orthopedic Journal of China Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Orthopedic Journal of China Year: 2006 Type: Article