Influence of gonadal hormones on the proliferation of lymphocytes in systemic lupus erythematosus (SLE) patients and the related mechanism / 中国免疫学杂志

ABSTRACT

Objective:To study the influence of gonadal hormones on the function of lymphocytes of SLE patients through detecting the expression of estrogen receptor alpha(ER?) and the function of peripheral blood lymphocytes of SLE patients.Methods:Blood samples of 33 SLE patients and 31 normal controls were collected.Estradiol(E2) and testosterone (T) concentration in peripheral blood were detected by ELISA,the proliferating reaction of T lymphocyte stimulated by ConA were detected by methyl thiazolyl tetrazolium (MTT) assay and the expression of estrogen receptor alpha(ER?)mRNA of peripheral blood mononuclear cells (PBMCs),T lymphocytes and B lymphocytes were detected by RT-PCR.Results:Compared with normal control,the stimulating index of T lymphocyte proliferation decreased(P