Effect of ultra-filtration extract from Danggui Buxue Decoction on spleen cells in H_(22) bearing mouse inducing IL-2 and IFN-? / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences)
;
(6)1982.
Article
in Chinese
| WPRIM
| ID: wpr-546680
ABSTRACT
Objective To evaluate the effect of ultra-filtration extract from Danggui Buxue Decoction (DBD) on immune function of H22 bearing mouse and explore the mechanism through observing the effect of ultra-filtration extract from DBD on the secretion of the cytokines (IL-2,IFN-?).Methods We used membrane separation technique to extract DBD.The content of IL-2 and IFN-? in splenocyte culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA),and the mRNA expression of cytokines (IL-2,IFN-?) in splenocytes was assayed by reverse transcription-polymerase chain reaction ( RT-PCR).Results The content of IL-2 and IFN-? in splenocyte culture supernatant was increased in ultra-filtration extract from DBD groups,significantly in high-dose group (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Xi'an Jiaotong University(Medical Sciences)
Year:
1982
Type:
Article
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