Adipose tissue derived stem cells transferred with TGF-?_1 gene and differentiated into chondrocytes / 中国矫形外科杂志
Orthopedic Journal of China
;
(24)2006.
Article
in Chinese
| WPRIM
| ID: wpr-546697
ABSTRACT
[Objective]To induce TGF-?1 gene which can increase ECM synthesis into adipose tissue derived stem cells(ADSCs) by employing gene transfer techniques and observe whether TGF-?1 gene could expresse continuously and whether type II collagen and aggrecan are synthesized in order to provide experimental data for constructing tissue-engineering cartilage. [Method]ADSCs were transferred with TGF-?1 gene ,TGF-?1,FN,ColⅡ and aggrecan were detected with RT-PCR and TGF-?1 protein was detected with Western-blot.[Result]RT-PCR demonstrated that the expression of TGF-?1,FN,ColⅡ and aggrecan in the TGF-?1 gene transferred groups increased more evidently than non-gene groups and control groups (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Orthopedic Journal of China
Year:
2006
Type:
Article
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