Preparing of biphasic ceramic biologic active bone BCBB/BMP/bFGF and the study of its biocompatibility with rabbit bone mesenchymal stem cells / 中国矫形外科杂志

ABSTRACT

[Objective]To prepare a kind of biphasic ceramic biologic active bone and study its biocompatibility.[Method]Biphasic ceramic biologic bone(BCBB) was mixed with collage type I,bone morphogenetic protein(BMP) and basic fibroblast growth factor(bFGF),and then the third generation cultured rabbit bone mesenchymal stem cells(MSCs) were seeded on BCBB/BMP/bFGF in vitro.The tissue engineering bone(BCBB/BMP/bFGF/MSCs) was observed by upside down microscope,scanning electron microscope(SEM) and examined using methylthiazol tetrazolium(MTT).[Result]Biphasic ceramic biologic active bone BCBB/BMP/bFGF was successfully prepared by reconstituting collage type I,BMP,bFGF and BCBB together.The rabbit cells grew in and on the BCBB/BMP/bFGF to form an ideal tissue engineering bone,and the cells quantity was the most on the 6th day.[Conclusion]BCBB/BMP/bFGF possesses a good biocompatibility with rabbit bone mesenchymal stem cells.