Effect of VEGF-C Gene Transfection on Expression of VEGF-C in Human Breast Cancer MCF-7 Cell / 中国普外基础与临床杂志
Chinese Journal of Bases and Clinics in General Surgery
;
(12)2008.
Article
in Chinese
| WPRIM
| ID: wpr-546862
ABSTRACT
Objective To explore the effect of vascular endothelial growth factor-C (VEGF-C) gene transfection on the expression level of VEGF-C in human breast cancer MCF-7 cell. Methods The constructed VEGF-C gene eukaryotic expression vector was transfected into the human breast cancer MCF-7 cell by using lipofectamine transfection reagents, and the positive cell clones were obtained through G418 selection after transfection. The expressions of VEGF-C mRNA and protein were detected by RT-PCR and Western blot respectively. Results Following the transfection of the VEGF-C recombination plasmid, there were significant differences on the expression levels of VEGF-C mRNA and protein between pcDNA3.1-VEGF-C transfection group and pcDNA3.1 transfection group (12.382?2.183 vs 6.039?1.950, P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Bases and Clinics in General Surgery
Year:
2008
Type:
Article
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