Histological and biomechanical study of deep-frozen allogenetic tendons / 中国矫形外科杂志

ABSTRACT

[Objective]To explore the allograft,as a substitute for autograft,whether can be used to repair the defect of the tendon and restore the stability of joint and the dynamic biomechanical changes of allograft after transplantation.[Method]Deep-frozen allogeneic achilles tendons of rabbits were used as treatment group,and autogenous ones as control.The achilles tendon defects of back limbs were repaired by allograft and autograft separately.The macroscopic,microscopic observation and biomechanical test were performed on both of them before transplantation and in 2,4 and 8 weeks after transplantation.[Result]It was showed that there were no differences in macroscopy,microscopy and mechanical strength between allograft and autograft both before and after transplantation.They may have experienced the similar healing course.The mechanical strength of allograft(except failure strain) was reduced significantly after transplantation.But it had a rising trend with time passing,although it was fairly low in 8 weeks compared with the normal such as maximum load.[Conclusion]The results demonstrate that the deep-frozen allograft can substitute for autograft in repairing the tendon defect,and because of the weakness of allograft after transplantation,it needs appropriate protection to prevent failure by excessive strain in early stage.