DNA-Protein Crosslink Induced by Formaldehyde and Repair in Pulmonary and Renal Cells of Rats / 环境与健康杂志

ABSTRACT

Objective To explore the DNA-protein crosslink(DPC) induced by formaldehyde(FA) and the repair.Methods Three-month-old Wistar male rats were used as the experimental animals and exposed to gaseous FA at the doses of 0,0.5,1.0,3.0 mg/m3 respectively through continuous inhalation for 72 h.The cellular suspension of the lungs and kidneys was exposed to FA at the doses of 0,25,50,100,150 and 200 ?mol/L respectively for 1 h.The KCl-SDS assay was used to detect the coefficient of DPC in the lung and renal cells.The repair at 0,6,12,18 and 24 h exposure points was determined in the exposure test of 3.0 mg/m3 for 72 h in vivo and 75 ?mol/L for 1 h in vitro.Results Higher gaseous FA(≥1.0 mg/m3) could significantly cause DPC(P