Study on Didymus Lipid Peroxidation Level on Rats after Sub-Acute Exposure to Di-2-ethylhexyl Phthalate / 环境与健康杂志

ABSTRACT

Objective To study genital toxicity and didymus lipid peroxidation level on rats after sub-acute exposure to di-2-ethylhexyl phthalate(DEHP).Methods The weaning Wistar andro-rats were dividied into four group randomly,and there were 10 rats in each group.Compared with the corn oil control,the experiment groups were gavaged consecutively for 30 days with the DEHP dose of 10,100,1 000 mg/(kg.d),The change of body weight was observed dynamically.All rats were executed by decapitation four weeks later and the didymium was removed immediately,at the same time viscera coefficient were measured.The level of MDA were detected by TBA method,meanwhile GSH level and GSH-Px activity were analyzed by modified DTNB method,the activity of SOD were measured by xanthine oxidase method.Results With the increasement of DEHP concentration,the body weight growth of rats in 1 000 mg/(kg.d) DEHP group is restricted compared with the control(P