Effect of constant magnetic field on metal ion Co~(2+),Cr~(3+) induced TNF-? secreted by human mononuclear cells / 中国矫形外科杂志
Orthopedic Journal of China
;
(24)2006.
Article
in Chinese
| WPRIM
| ID: wpr-547361
ABSTRACT
[Objective]To detect the effect of constant magnetic field on metal ion Co2+,Cr3+ induced TNF-? secreted by human mononuclear cells,and to search a method for prevention and treatment of aseptic loosening. [Methods]CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mononuclear cells from human peripheral blood,were taken and cultured with Co2+,Cr3+ ions in different magnetic field of 10Gs,100 Gs,1000Gs for 12,24 and 48 hs. There were nine groupscontrol group,Co2+ group,Cr3+ group,Co2+andCr3+ with various intensities of constant magnetic field,respectively.ELISA method was applied to detect tumor necrosis factor (TNF-?) in serum via the absorbance (A).[Results]Co2+ and Cr3+ ions stimulated human mononuclear cell to secrete TNF-? (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Orthopedic Journal of China
Year:
2006
Type:
Article
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