Effects of Lanthanum on Cell Multiplication and Intracellular Free Ca~(2+) Concentration in Primary Cultured Astrocytes of Rats / 环境与健康杂志
Journal of Environment and Health
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-547509
ABSTRACT
Objective To study the effects of lanthanum on cell multiplication and intracellular Ca2+([Ca2+]i) concentration in primary cultured astrocytes of rats.Methods The primary cultured astrocytes of rats were exposed to 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h.The cell multiplication was measured by MTT;[Ca2+]i concentration was determined by the fluorescent Ca2+ indicator Fura-2/AM.Results After astrocytes were incubated with 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h,the survival rate of astrocytes was 86.24% and 82.99%,82.26% and 79.14%,52.32% and 49.97%,38.04% and 35.15% respectively,which was significantly decreased compared with the control group(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Environment and Health
Year:
1992
Type:
Article
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