Saturated fatty acids trigger apoptosis and inhibit cell cycle progression in insulinoma cells / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the effect of saturated fatty acid palmitate acids(PA) action on viability,proliferation and apoptosis of murine insulinoma MIN6 cells,and the possible intracellular pathways activated by PA.Methods Cell viability was evaluated with MTT reduction conversion assay.The Annexin-Ⅴ/FITC cell death detection kit was used to monitor PA-induced apoptosis.(IAP) family(XIAP and cIAP-2),cyclin D1 and CDK4 were further detected using Western blot.Results ① PA of different concentration significantly inhibited the proliferation of MIN6 cells(P