Anti-tumor effect of cytosine deaminase genetherapy in situ combined with thermochemotherapy for colon cancer liver metastasis in nude mice / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
;
(4)2009.
Article
in Chinese
| WPRIM
| ID: wpr-547736
ABSTRACT
Objective:
To investigate the treatment effect of cytosine deaminase gene combined 5-FC thermochemotherapy for colon cancer liver metastasis in Nude Mice.Methods:
The models of liver metastasis of colon cancer had been established by injecting human colon cancer cells Lovo into portal vein in 30 BALB/c nude mice,which were divided into transgenic group and non-transgenic group randomly. Transgenic groupvirus supernatant was injected into abdominal cavity(0.2 mL/d,for 5 days); non-transgenic groupsodium chloride was injected into abdominal cavity(0.2 mL/d,for 5 days). In both groups 42 ℃ 5-FC was injected into the abdominal cavity (500 mg?kg-1?d-1,for 21 days). After treatment,all mice were sacrificed,and then PCR and RT-PCR were performed to detect the expression of targeted gene in carcinoma. The pathology changes of tumor were observed.Results:
The targeted gene were detected in the tumor tissues. The rates of liver metastasis tumors were 26.7%(4/15),100.0%(15/15) in transgenic group and non-transgenic group respectively(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Current Advances in General Surgery
Year:
2009
Type:
Article
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