PATHOLOGICAL STUDY OF TOXIC EFFECT OF AFLATOXINB_1 ON RAT LIVER / 第二军医大学学报

ABSTRACT

Thirty one male Wistar rats weighing 130-150 g were continuously fed with aflatoxin B1 (AFB1) for 45 weeks.Each rat received 3.89mg of AFB1 crystals in total close.Three treated rats and one control ret were killed at the 8th, 9th, 11th, 12th, 13th, 14th and 1.3th month, respectively, and seven rats died naturaly after the 17th month.Histological sections of the liver were stained with hematoxylin-eosin.Morphological changes of 28 rats' liver tissues stained with enzyme-labelled immuno-fetoprotein, and ultrastructural chinges of the liver specimens from 15 rats were observed .Histologicall ', the main manifestations of liver cells included hydratoid degeneration, eosinophilic degeneration, basophilic change.adipose degeneration and focal necrosis.The answer reaction showed oval cell and bilbary proliferation, hepatocellular regeneration and inflammatory cell infiltration.All of the above changes were chronic non-specific toxic hepatitis. The ultrastructural changes showed nucleolar uniformation, nucleoid body formation, mitochondria swelling and matrix uniformation, and vesicle formation and focal necrosis. 85.71 % 24/28)of the alpha-fetoprotein positive hepatocytes were found in host liver tissues.The AFP positive material mostly distributed in all of the plasma of liver cells.AFP positive hepatocytes were dispersed or focal in the liver tissue. This result suggests that the liver cell atavism mav be the toxic effect of aflatoxin B1.