Role of Nuclear Factor-kappa B Activation in Inflammatory Effect Induced by PM2.5 from Beijing Urban Area in A549 Cells / 环境与健康杂志

ABSTRACT

Objective To study the adverse effects of PM2.5 from Beijing urban areas on human lung adenocarcinoma cells(A549) and the expression of NF-?B in the cells. Methods A549 cells were treated with PM2.5 at 25,50,100 and 200 ?g/ml for 24 h. Cytotoxicity of PM2.5 was measured by MTT assay. The activity of NF-?B was measured by ELISA assay. Western blot method was used to detect the expression of NF-?B and NO levels was determined by using the nitrate reductase method. Results PM2.5 could induce A549 cell proliferation at low doses,but inhibit cell proliferation at high doses. The activity of NF-?B increased in the cell nucleus,but decreased in the cytoplasm after exposure to PM2.5 with a significant dose-dependent manner(P