Effects of Airway Inflammation Induced by Low Level Ozone and Nitrogen Dioxide Exposure in Independent and Combined in Mice / 环境与健康杂志

ABSTRACT

Objective To investigate the effects of single and combined exposure to ozone and nitrogen dioxide on airway injury and inflammatory levels in mice. Methods Eighty BALB/C mice were randomly divided into eight groupsfour were sensitized model groups(the control group,the O3 group,the NO2 group and the O3+NO2 group based on the way of exposure) ,the other four were non-sensitized groups. The concentration of O3 was 0.16 mg/m3,NO2 was 0.30 mg/m3. Mice were exposed 2 hours every day for 7 consecutive days. Mice were sacrificed in the exposure end and cell counting and cytokine measurement from bronchoalveolar lavage fluid(BALF) were performed. Results In the sensitized groups,neutrophil proportion and IL-6 were significantly increased except the control group and IL-2 and MDA were also significantly increased in the O3 group and the O3+NO2 group(P