Role of Src kinase in the induction and maintenance of spinal long-term potentiation / 西安交通大学学报(医学版)

ABSTRACT

Objective To evaluate the role of Src kinase in the induction and maintenance of spinal long-term potentiation(LTP).Methods The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement.Results ① Genistein(200?mol/L) or PP2(100?mol/L),a selective Src kinase inhibitor,completely blocked LTP induction when administered at 30min prior to tetanic stimulation.② Genistein or PP2 reversed spinal LTP in a time-dependent manner.At 15min after LTP induction,Genistein(200?mol/L) or PP2(100?mol/L) reversed LTP completely.At 30min after LTP induction,however,the same concentration of Genistein or PP2 did not affect the spinal LTP.Conclusion Activation of Src kinase in spinal dorsal horn may be crucial for the induction and early-phase maintenance of LTP of C-fiber evoked field potentials.