Changes of Protein Concentration (Cpro) and Nitric Oxide Synthase (NOS) of Serum,Heart,Lungs,Brain and Liver in Acute Pulmonary Edema Rats Model Induced by Formaldehyde Inhalation / 环境与健康杂志

ABSTRACT

Objective To study the changes of the protein concentration (Cpro) and nitric oxide synthase (NOS) of serum, heart, lungs, brain and liver in acute pulmonary edema rats model induced by formaldehyde inhalation, to provide data for studying in acute pulmonary edema animal model induced by formaldehyde inhalation. Methods Eighteen SD female rats were randomly divided into the control group and the model group, and the model group rats were inhaled vaporous formaldehyde in a self-made cabinet for 2 hours and were sacrificed 8 hours later. The blood sampling, lungs, heart, brain and liver were collected and lungs/ heart index, heart/body weight index and lungs/body weight index were calculated, NOS were detected with NOS kit and protein determination kit, and the pathological examination of lungs tissue was done. Rats of control group were treated as the same as model group, but formaldehyde was replaced by normal saline. Results Compared with the control, lungs/heart index increased significantly (P0.05). Conclusion In acute pulmonary edema rats induced by formaldehyde inhalation, the protein concentration of serum, heart, brain and lungs tissues increase significantly, NOS does not change obviously.