Antagonism of N-acetylcysteine to Energy Metabolism Damage of Renal Cortical Mitochondria Induced by Mercury in Rats / 环境与健康杂志

ABSTRACT

Objective To explore the effect of HgCl2 exposure on energy metabolism of renal mitochondria in rats and the preventive effect of N-acetylcysteine(NAC).Methods Twenty-four Wistar rats were randomly divided into control group,HgCl2 group and NAC+HgCl2 group by body weight,8 in each.Control group and HgCl2 group were treated with physiological saline through intraperitoneal injection,NAC+HgCl2 group rats were intraperitoneally pretreated with NAC(0.4 mmol/kg),two hours after treatment,HgCl2 group and NAC+HgCl2 group were injected subcutaneously with HgCl2(1.84 ?mol/kg),but control group was injected with physiological saline,the volume of was 5 ml/kg,once a day for 14 consecutive days.Twenty-four hours after the last injection,all rats were sacrificed and the cortices of rat kidneys were collected.The mitochondria were prepared by using the differential centrifugation.The levels of malondialdehyde(MDA),the activities of phospholipase A2(PLA2) and succinate dehydrogenase(SDH),the mitochondrial membrane potential were measured.Results Compared with control group,the level of MDA and the activity of PLA2 significantly increased in HgCl2 group(P