Effect of ?-rays on the histomorphology and hydroxyproline content of human tendon / 中国矫形外科杂志

ABSTRACT

[Objective]To study the changes of the histomorphology and content of hydroxyproline that irradiated by 2.87 Mrad ?-rays in human tendon. [Methods]Forty fresh human tendons were freezed in-80℃ for six weeks,and randomly divided into two groups the experimental group(group A) and control group(group B).Group A stored in dry ice was exposed to ?-rays until the absorbed dose reached 2.87 Mrad.The tendons was stained with HE and VG methods,and then observed by common light microscope and transmission electron microscope respectively.The content of hydroproline in tendon collagen hydrolysate was determined by the high performance liquid chromatography with precolumn derivation.[Results]①The results of histomorphology observation revealed that compared to the control group,the gaps between collagen fiber bands in irradiation group were enlarged,incompact,disordered and some of them fractured.The electron microscope observation showed that the transverse striation of the collagen fibers became unclear or disappeared.The membrane of tendon cell was dissolved,the cell nuclear was disintegrated and the organelles was reduced.②The content of hydroxyproline in the collagen hydrolysate of irradiation group was significantly higher than that in non-irradiation group under the same hydrolysis condition(P