Establishment of a mouse model of Humulus pollen allergen-induced allergic asthma / 西安交通大学学报(医学版)

ABSTRACT

Objective To develop a mouse model of allergic airway inflammation by using crude Humulus pollen extract.Methods A total of 24 Balb/c mice were divided randomly into two groups control group and asthma model group with 12 in each.After sensitization by intraperitoneal injection,the mice were challenged by intranasal instillation of crude Humulus pollen extract.The total cell number and eosinophile percentage of bronchoalveolar lavage fluid(BALF) samples were determined.The lungs of mice were stained with hematoxylin and eosin to evaluate the degree of allergic inflammation.The cytokines in BALF and spleen homogenate were assayed by enzyme-linked immunoassay(ELISA).The total IgE in the serum was also measured by ELISA.Results Compared with the mice in normal control group,those in asthma group developed obvious allergic inflammation in the airways.Histological scoring for pulmonary inflammation significantly differed between normal group and asthma group(P