Study on targets for several traditional medical formula in innate immunity against M.tuberculosis / 中国免疫学杂志

ABSTRACT

Objective:To explore the potential targets for Traditional Chinese Medical Formula against tuberculosis infection.Methods:U937 cells were incubated with the drug-serum prepared with Clehnia root Ophioponis Decoction(COD),Lily Metal-Consolidating Decoction (LMCD) and Radix Ginseng Gecko Powder(RGGP) for 24 hours.The mRNA and protein level of CR1,CR3,CD14,CD43,TLR2 and TLR4 expressed in macrophages respectively were then measured by RT-PCR and FACS.The phagocytic and killing activity of the U937 cells interfered with the drug-serum for 24 h were then detected after BCG stimulation.Meanwhile,secretion of IL-10,IL-12 and the activity of iNOS in the macrophages were detected by ELISA and biochemistry method in each group.Results:The drug-serum prepared with COD,LMCD and RGGP had different regulatory effects on the expression of PRRs and the production of IL-10 and IL-12 in the macrophages,and could enhance the phagocytosis percentage and phagocytic index(P