A PRELIMINARY REPORT OF THE EXPERIMENTAL STUDY ON THE EFFECT OF HPD AND LASER ON HUMAN HEPATOCARCINOMA CELL LINE / 第二军医大学学报

ABSTRACT

The present article reports the observation of the effect of HPD and laser on human hepatocarcinoma cells.The experimental result shows that HPD (50?g/ml) does not influence the growth of the hepatocarcinoma cell line,and laser alone for as long as 20 minutes does not bring about any changes of the shape of cell. Cyto-logical damage occurrs when the HPD culture medium is irradiated with lasers.A remarkable killing effect on the human hepatocarcinoma cell line is shown along with increasing HPD concentration and laser power.In order to distinguish the degree of cell damage, we suggested that changes of cell shape could be classified into three degrees.It was discovered in the experiment that most of cultured cells developed degeneration and necrosis after being treated with HPD plus laser but still there were some islands of cells alive.Authors considered that this deficiency might be unevenness in the distribution of laser action to account for the relapse of some treated cases and accordingly suggested that in the HPD treatment plus laser, the order of distribution of the irradiation should be well plotted in order to obtain better therapeutic effect.