A New Driving- System for Microelectrode in Animal Experiments / 第三军医大学学报

ABSTRACT

This paper is to introduce our newly designed driving system used to push a microelectrode forward into the recording position in animal experiments. The instrument consists of two parts 1. the hydraulic fine manipulator and 2. the holding mechanism and the secrew driving device of the microelectrode.Carefully pass a long glass shaft microelectrode through a stainless steel guide tube. Both the microelectrode and the guide tube are fixed on the frame of the instrument with two holding mechanisms. The frame is then connected to the chassis of the instrument which has previously been embedded in the skull of the experimental animal. Turn the screw driving device and both the microelectrode and the guide tube are moved forward. Stop turning when the guide tube penetrates the dura. Then the hydraulic fine manipulator is used to push the microelectrode into the recording position.A long glassshaft microelectode produces little injury to the brain tissue, thus it facilitates the repeated experiments on animals. The chassis of the driving system is implanted into the skull of the experimental animal with stereoscopic technique and its relative position with the brain tissue can not be changed during experiment. Slight movement of the animal head will in no way interfere with the recording.