Enzyme-linked Immunosorbent Assay to Determine Specific Immune Complex in Rabbit circulation / 第三军医大学学报

ABSTRACT

A method, enzyme-linked immunosorbent assay, was introduced for the determination of antigen-specific immune complex (BSA-anti-BSA) in rabbit circulation. The antigen-specific immune complex in the circulation of twelve rabbits were determined by this method. The results showed that this method accords with the regular pattern of immune complex formation more closely than the antigen-nonspecific assay, PEG precipitation method. The procedure of this method is rather simple, its specificity is of high degree, and the amount of serum needed in test is small. This enzyme-linked immunosorbent assay is a better method to determine the circulating specific immune complex in rabbits.