AN INDIRECT PEROXIDASE ANTIBODY TEST (IPA) FOR DETECTION OF ANTIBODIES TO HEMORRHAGIC FEVER WITH RENAL SYNDROMES (HFRS) / 解放军医学杂志

ABSTRACT

Hemorrhagic fever with renal syndrome (HFRS) occurs endemically in certain localities in our country.At present, the diagnostic method usually used is indirect flurorescent antibody (IFA) technique, but due to the requirement of costly equipments and trained technicians, it is not feasible in grass-root clinics. We used an indirect peroxidase antibody method (IPA) to detect HFRS specific IgM. and their results were compared with that of IFA. It was found that the IPA titre was 2.6 times higher than IFA in sera of early cases, and 20 times higher than IFA titers in convalescent sera. Of the 49 sera tested. 40 gave a positive result by IPA. 35 positive by IFA. There was discrepancy between IPA and IFA in 1 serum. Only ordinary light-microscope is necessary to perform IPA test, and the test is sensitive, specific, and easy to perform. The procedure is simpler and can be used in grass-root medical units