THE ESTIMATION OF ?-TOCOPHEROL IN HUMAN BLOOD BY GAS CHROMATOGRAPHY / 营养学报

ABSTRACT

The estimation of a-tocopherol in human blood was carried out by gas chromatography. The cholesterol in plasma must he removed in advance. The a-tocopherol is chromatographed as tocopherol acetate and is eluted with a retention time of about 8 min. Tetratriacontane was used as internal standard. The average recovery rate was 76.4 per cent and coefficient of variation was 4.4 per cent (N = 10). The mean level in 25 cord blood was 2.1 ?0.5 ?g/ml. It correlated well with the results by spectrophotome-tric method (r=0.6803 P