Efficacy of DMAP Injection in Treatment of HCN Poisoning in Dogs / 第三军医大学学报

ABSTRACT

The toxicity of HCN was determined first.The experimental animals were intoxicated with a HCN concentration of 1103.8?48.32 Y/L and were exposed for 4.55?2.04 minutes.The mortality rate in 24 hrs was recorded.The LDEO,sLD50,and LD99 were calculated as 844.31?/kg,172.39?/kg and 959.18?/kg respectively.Two preparations of DMAP injection were found to have preserved rather good anti-HCN potency even though they had been kept under room temperature for about one year.All the 11 dogs survigd a dose of 4 LD50 of HCN in halation poisoning when DMAP was injected intramuscularly 1 minute after exposure.Another 5 dogs inflicted with similar HCN intoxication were also all saved when intravenous DMAP injection was given at the time of respiratory arrest. The cyanide level in the blood of the dogs after inhalation of HCN was determined by spectrophotofluorometry with pyridoxal.The blood concentration after inhalation of a lethal dose of HCN was 110?26.6?/100 ml at the moment of respiratory arrest.DMAP after intramuscular injection can shift the cyanide combined with cytochrome oxidase to red blood cells to form ferrihemoglobin cyanide,and cell respiration is then restoredEventually the dogs could survive the 4 LD50 HCN intoxication.