SIMULTANEOUS DETERMINATION OF VITAMIN B_1, B_2 AND NIACIN IN FORTIFIED MIXED PROTEIN BY PAIRED-ION REVERSED-PHASE HIGH PERFORMANCE CHROMATOGRAPHY / 营养学报

ABSTRACT

A high-performance liquid chromatographic procedure for the simultaneous determination of vitamin B1, B2 and niacin in fortified mixed protein was developed. The sample was heated on water bath by adding 0.1N HC1 at 100℃ for 30 min. Afterward, the vitamins in the filtrate of the sample were separated on a column of u-bondapak C18 with methanol/water containing 0.005M pic B7 ion pair reagents (4060 V/V) as mobile phase, and then the eluate was measured at 254nm. The detection limits for vitamin B1, B2 and niacin were approximately 15 ng, 5ng and 6ng respectively. The average recoveries of vitamin B1, B2 and Niacin were 92.21,90.25 and 92.33% respectively. The coefficients of variation of these vitamins were all under 6%. The method is simple, rapid and accurate, so it could also be applied to the analysis of other samples such as fortified foods and multivitamin preprati-ons etc.