A Study of Lipid Peroxide Contents in Tissues after Burns in Rats. Dynamic Changes of Lipid Peroxide and Vitamin E in Plasma, Eschar and Liver Tissue / 第三军医大学学报

ABSTRACT

20% TBSA full thickness burns was inflicted on the back of rats with a 5 KW bromotungsten lamp. The animals were sacrificed on the 1st, 2nd, 3rd, 5th, 7th, and 9th day post-burn. Then the lipid peroxide and Vitamin E contents of the plasma, eschar and liver homogenate, mitochondria and microsomes were determined. It was found that the lipid peroxide content of the plasma and eschar increased postburn. The elevation of lipid peroxide level in the escbar showed 2 peaks on the 2nd and 7-9th day postburn and was related to the positive bacterial culture of the sube-schar tissues. The changes of the lipid peroxide levels in the mitochondria and microsomes were similar to those in the eschar. The elevation of plasma lipid peroxide showed only one peak on the 3rd day postburn. The vitamin E levels in these tissues decreased markedly after injury, reached the lowest point 3-5 days after burns, and maintained at a low level throughout the whole course of observation.The causes of the increase of lipid peroxidation and its damage to the subcel-lular structures were briefly discussed.